Anti-dimethyl Histone H4 (Arg3); Asymm

Code: ABE441 D2-231

Application

Research Sub CategoryHistones

This Anti-dimethyl Histone H4 (Arg3) antibody, Asymmetric is validated for use in western blotting, ICC & dot blot for the det...


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€396.00 EACH
€487.08 inc. VAT

Application

Research Sub CategoryHistones

This Anti-dimethyl Histone H4 (Arg3) antibody, Asymmetric is validated for use in western blotting, ICC & dot blot for the detection of dimethyl Histone H4 (Arg3).

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected dimethyl Histone H4 (Arg3), Asymmetric in HeLa cells.

Dot Blot (Specificity) Analysis: A 1:10,000 dilution from a representative lot detected dimethyl Histone H4 (Arg3), Asymmetric in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

DyLight™ is a registered trademark of Thermo Fisher Scientific.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The histones have an amino terminal tail, a globular domain, and a carboxy-terminal tail. The four core histones, H2A, H2B, H3 and H4 assemble into the octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped in a sequence-independent manner around the octamer, forming the basic subunit of chromatin, the nucleosome. Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Immunogen

KLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4, asymmetrically dimethylated at Arg3.

Epitope: N-terminus

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in untreated and CARM1 treated recombinant Histone H4.

Western Blotting Analysis: A 1:50,000 dilution of this antibody detected dimethyl Histone H4 (Arg3), Asymmetric in 0.5 µg of CARM1 treated recombinant Histone H4.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~13 kDa observed

antibody formaffinity isolated antibody
antibody product typeprimary antibodies
biological sourcerabbit
clonepolyclonal
Gene Informationhuman ... HIST2H4B(554313)
NCBI accession no.NP_001029249
purified byaffinity chromatography
Quality Level100
shipped inwet ice
species reactivityhuman
technique(s)immunocytochemistry: suitable, western blot: suitable, dot blot: suitable
UniProt accession no.P62805
This product has met the following criteria to qualify for the following awards:



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